Ethidium bromide CAS NO.1239-45-8

NAME OF MATERIAL:- HOMIDIUM BROMIDE BP CAS NO- 1239-45-8 M.WT. 394.33 MOL FORMULA: - C21H20BrN3 Sr.No Tests Specifica…

NAME OF MATERIAL:- HOMIDIUM BROMIDE BP CAS NO- 1239-45-8 M.WT. 394.33 MOL FORMULA: - C21H20BrN3 Sr.No Tests Specifications Results 01 Description Dark Red to purple Colored Powder Complies 02 Solubility Soluble in 20 Parts of water. Complies 03 Identification a) IR – Similar to standard Complies b) U.V. Similar to standard Complies c) 2-naphthol test gives intense dark blue colour Complies 04 Loss On drying NMT 5 % 3.11% 05 Melting point. 236 to 240 deg. C And decomposition at 260 to 262 deg. cel. 238 deg .c 06 Ph 2. % w/v, in water 4 to 7 5.77 07 Related Substances TLC Test Passes Passes 08 Assay NLT. 95 % 98.25% Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis